Muscle plasticity related to changes in tubulin and alpha B-crystallin levels induced by eccentric contraction in rat skeletal muscles

作者：Jee H; Ochi E; Sakurai T; Lim J Y; Nakazato K; Hatta H^*

来源：Physiology International, 2016, 103(3): 300-309.

DOI：10.1556/2060.103.2016.3.4

• heat shock protein
• cytoskeleton
• skeletal muscle
• myosin heavy chain
• isoform type transition
• electrically induced eccentric contraction

We used the model of eccentric contraction of the hindlimb muscle by Ochi et al. to examine the role of eccentric contraction in muscle plasticity. This model aims to focus on stimulated skeletal muscle responses by measuring tissue weights and tracing the quantities of alpha B-crystallin and tubulin. The medial gastrocnemius muscle (GCM) responded to electrically induced eccentric contraction (EIEC) with significant increases in tissue weight (p < 0.01) and the ratio of tissue weight to body weight (p < 0.05); however, there was a decrease in soleus muscle weight after EIEC. EIEC in the GCM caused contractile-induced sustenance of the traced proteins, but the soleus muscle exhibited a remarkable decrease in alpha-tubulin and a 19% decrease in alpha B-crystallin. EIEC caused fast-to-slow myosin heavy chain (MHC) isoform type-oriented shift within both the GCM and soleus muscle. These results have shown that different MHC isoform type-expressing slow and fast muscles commonly undergo fast-to-slow type MHC isoform transformation. This suggests that different levels of EIEC affected each of the slow and fast muscles to induce different quantitative changes in the expression of aB-crystallin and alpha-tubulin.

• 出版日期2016-9