Acetylation and deacetylation for sucralose preparation by a newly isolated Bacillus amyloliquefaciens WZS01

作者：Sun, Jie; Chen, Liang; Lou, Bo; Bai, Yanbing; Yu, Xinjun; Zhao, Man; Wang, Zhao^*

来源：Journal of Bioscience and Bioengineering, 2017, 123(5): 576-580.

DOI：10.1016/j.jbiosc.2016.12.013

• Bacillus amyloliquefaciens
• Sucralose
• Sucrose-6-acetate
• Acetylation
• Deacetylation

Sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. In the present study, Bacillus amyloliquefaciens WZS01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. Bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. The yield of sucrose-6-acetate was >95% with 60 mM sucrose after 22 h of reaction. Free cells could hydrolyze 75 mM sucralose-6-acetate to produce sucralose with >99% yield after 24 h of reaction. B. amyloliquefaciens WZS01 could be considered a potential biocatalyst for sucralose preparation.

• 出版日期2017-5
• 单位浙江工业大学