Disruption of the Actin Cytoskeleton Induces Fluorescent Glucose Accumulation on the Rat Hepatocytes Clone 9

作者：Espina Begona; Carmen Louzao M; Ares Isabel R; Fonfria Eva S; Vilarino Natalia; Vieytes Mercedes R; Botana Luis M^*

来源：Cellular Physiology and Biochemistry, 2011, 27(6): 653-660.

DOI：10.1159/000330074

• 2-NBDG
• Clone 9 hepatocytes
• Confocal microcopy
• F-actin
• Glucose metabolism

Background: Glucose transport and metabolism are highly specialized in hepatocytes. Actin cytoskeleton is fundamental to the maintenance of their morphology as well as to ensure their functionality. Here we study the effect of the actin disrupting natural compounds cytochalasin B and latrunculin A on the glucose metabolism of the Clone 9 rat hepatocytes once the glucose molecule is inside them and the effects of two hormones which main function is regulating the glucose metabolism on the actin cytoskeleton of Clone 9 cells. Methods: F-actin was labeled by using Oregon Green 514 (R) phalloidin and glucose inside cells was monitored with the fluorescent D-glucose derivative; 2-NBDG. Observations and measurements were carried out by using a confocal microscope. Results: Nor insulin neither glucagon was able to induce any significant effect in the quantity of F-actin present on Clone 9 cells. But insulin triggers a strong reorganization on the pattern of distribution of F-actin. However, the actin cytoskeleton disruption induced by CB and more efficiently by Lat A caused accumulation of 2-NBDG in cells. Conclusions: These results state that disruption of the actin cytoskeleton induces fluorescent glucose accumulation on the rat hepatocytes Clone 9 suggesting that actin disrupting agents cause a blockage in the glycolytic pathway of Clone 9 hepatocytes.

• 出版日期2011