High-Content Analysis of Breast Cancer Using Single-Cell Deep Transfer Learning

作者：Kandaswamy Chetak^*; Silva Luis M; Alexandre Luis A; Santos Jorge M

来源：Journal of Biomolecular Screening, 2016, 21(3): 252-259.

DOI：10.1177/1087057115623451

• high-content screening
• image analysis
• deep transfer learning
• cancer drug discovery

High-content analysis has revolutionized cancer drug discovery by identifying substances that alter the phenotype of a cell, which prevents tumor growth and metastasis. The high-resolution biofluorescence images from assays allow precise quantitative measures enabling the distinction of small molecules of a host cell from a tumor. In this work, we are particularly interested in the application of deep neural networks (DNNs), a cutting-edge machine learning method, to the classification of compounds in chemical mechanisms of action (MOAs). Compound classification has been performed using image-based profiling methods sometimes combined with feature reduction methods such as principal component analysis or factor analysis. In this article, we map the input features of each cell to a particular MOA class without using any treatment-level profiles or feature reduction methods. To the best of our knowledge, this is the first application of DNN in this domain, leveraging single-cell information. Furthermore, we use deep transfer learning (DTL) to alleviate the intensive and computational demanding effort of searching the huge parameter's space of a DNN. Results show that using this approach, we obtain a 30% speedup and a 2% accuracy improvement.

• 出版日期2016-3