Uricase-Based Highly Sensitive and Selective Spectrophotometric Determination of Uric Acid Using BSA-Stabilized Au Nanoclusters as Artificial Enzyme

作者：Zhao Huihui; Wang Zhonghui; Jiao Xue; Zhang Lichun; Lv Yi^*

来源：Spectroscopy Letters, 2012, 45(7): 511-519.

DOI：10.1080/00387010.2011.649440

• au nanoclusters
• biological template
• peroxidase mimetics
• uric acid

The enzymatic spectrophotometric analysis of uric acid based on BSA-stabilized Au nanoclusters (Au NCs) as peroxidase mimetics was first developed. Compared with natural enzyme horseradish peroxidase (HRP), which was used widely to detect H2O2 generated by uric acid, the BSA-stabilized Au NCs as peroxidase mimetics are easy to prepare, low cost, and stable. Kinetic analysis indicates that the BSA-stabilized Au NCs have even higher catalytic activity than HRP. Under the optimum conditions, the detection limit for uric acid is 3.6 x 10(-7) mol L-1. The feasibility of the developed method for uric acid analysis in human serum was confirmed.

• 出版日期2012
• 单位四川大学