A switched catalysis qualified sealers capped one-step synthesis biocompatibility bimetallic scaffold film for Neu5Ac alpha(2-6)Gal beta MP Glycoside specific detection

作者：Xia, Chunyong; He, Junlin; Yuan, Guolin; Li, Yuliang; Li, Qingying; Yu, Chao^*

来源：Biosensors and Bioelectronics, 2016, 77: 853-859.

DOI：10.1016/j.bios.2015.10.060

• Neu5Ac alpha(2-6)Gal beta MP Glycoside
• AuPt-PPy
• Glucose oxide
• Electrochemical catalytic

In this work, a novel label-free biosensor was designed for the sensitive and selective determination of Neu5Ac alpha(2-6)Gal beta MP Glycoside using AuPt-PPy(polypyrrole) conductive nanocomposite film as the sensor platform. The introduced AuPt-PPy nanocomposite provided a large surface area for the immobilization of Sambucus nigra agglutinis (SNA) through a coupling agent for specifically recognizing analytes and exhibited high electrocatalytic activity toward the reduction of hydrogen peroxide (H2O2) as an analytical signal. Subsequently, to block the non-specific sites of the modified electrode, GOx was employed instead of the usual sealers. Most importantly, in the presence of glucose, these localized GOx further enhanced the electrochemical signal, which was achieved by the efficient catalysis of glucose. This study is the first that demonstrates the specific detection of Neu5Ac alpha(2-6)Gal beta MP Glycoside using AuPt-PPy as the electrocatalytic. Under optimal conditions, the electrochemical biosensor exhibited a wide linear range of 0.01 pg mL(-1)-800 ng mL(-1) with a low detection limit of 0.003 pg mL(-1) (S/N=3), due to the affinity between SNA and Neu5Ac alpha(2-6)Gal beta MP Glycoside. Therefore, the co-catalysis signal amplification approach has considerable potential in clinical applications and is suitable for the quantification of other biomarkers.

• 出版日期2016-3-15
• 单位重庆医科大学