Determination of ortho-phthalaldehyde in water by high performance liquid chromatography and gas chromatography-mass spectrometry after hydrazine derivatization

作者：Oh Jin Aa; Shin Ho Sang^*

来源：Journal of Chromatography A, 2012, 1247: 99-103.

DOI：10.1016/j.chroma.2012.05.065

• ortho-Phthalaldehyde
• Hydrazine derivatization
• High performance liquid chromatography
• Gas chromatography-mass spectrometry

A simple high performance liquid chromatographic (HPLC) method and a highly sensitive gas chromatography mass spectrometric (GC-MS) method have been established for the determination of ortho-phthalaldehycle (OPA) in water. These methods are based on the derivatization of OPA with hydrazine in water. The following optimum reaction conditions were established: reagent dosage. 20 mg/mL of hydrazine: pH 2: reaction for 20 min at 70 degrees C. The organic derivative was detected directly by HPLC or after the extraction with methylene chloride/concentration by GC-MS. The limit of detection of OPA in water was 4.0 and 0.3 mu g/L by HPLC and GC-MS, respectively. The calibration curve showed good linearity with r(2) = 0.9993 and r(2) = 0.9994 by HPLC and GC-MS, respectively, the accuracy was in a range of 95-105%, and the precision of the assay was less than 13% in water. The HPLC method was simple and reproducible enough to permit the OPA content analysis in the disinfectant products, and the GC-MS method is sensitive enough to permit reliable analysis of OPA to the mu g/L level in environmental water.

• 出版日期2012-7-20