Dynamin Participates in the Maintenance of Anterior Polarity in the Caenorhabdiffs elegans Embryo

作者：Nakayama Yuji; Shivas Jessica M; Poole Daniel S; Squirrell Jayne M; Kulkoski Jennifer M; Schleede Justin B; Skop Ahna R^*

来源：Developmental Cell, 2009, 16(6): 889-900.

DOI：10.1016/j.devcel.2009.04.009

Cell polarity is crucial for the generation of cell diversity. Recent evidence suggests that the actin cytoskeleton plays a key role in establishment of embryonic polarity, yet the mechanisms that maintain polarity cues in particular membrane domains during development remain unclear. Dynamin, a large GTPase, functions in both endocytosis an actin dynamics. Here, the Caenorhabditis elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. DYN-1-GFP foci are enriched in the anterior cortex in a manner dependent on the anterior polarity proteins, PAR-6 and PKC-3. Membrane internalization and actin comet formation are enriched in the anterior, and are dependent on DYN-1. PAR-6-labeled puncta are also internalized from cortical accumulationsof DYN-1-GFP. Our results demonstrate a mechanism for the spatial and temporal regulation of endocytosis in the anterior of the embryo, contributing to the precise localization and maintenance of polarity factors within a dynamic plasma membrane.

• 出版日期2009-6-16