A noncytotoxic DsRed variant for whole-cell labeling

作者:Strack Rita L; Strongin Daniel E; Bhattacharyya Dibyendu; Tao Wen; Berman Allison; Broxmeyer Hal E; Keenan Robert J; Glick Benjamin S*
来源:Nature Methods, 2008, 5(11): 955-957.
DOI:10.1038/nmeth.1264

摘要

A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

  • 出版日期2008-11