Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

作者：Liaudanskas Mindaugas^*; Viskelis Pranas; Jakstas Valdas; Raudonis Raimondas; Kviklys Darius; Milasius Arvydas; Janulis Valdimaras

来源：Journal of Chemistry, 2014, 2014: 542121.

DOI：10.1155/2014/542121

A specific analytical procedure including sample preparation and HPLC analysis was developed and validated for the detection of phenolic compounds in the samples of different apples from popular Lithuanian cultivars "Aldas," "Auksis," "Ligol," and "Sampion." The conditions for phenol extraction were optimized: the solvent of the extraction was 70% (v/v) ethanol, and the extraction was performed in an ultrasound bath for 20 min at the temperature of 40 degrees C. The HPLC mobile phase consisted of 2% (v/v) acetic acid in water and 100% (v/v) acetonitrile. Using the HPLC technique, 11 analytes were identified, and their specificity was confirmed: procyanidin B1, (+)-catechin, chlorogenic acid, procyanidin B2, (-)-epicatechin, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, and phloridzin. Chlorogenic acid was the major component in "Aldas," "Auksis," and "Ligol" and procyanidin B2 in "Sampion." Hyperoside and avicularin were the dominant compounds of all the identified quercetin derivatives in "Aldas" and "Auksis;" hyperoside in ".Sampion;" and quercitrin in "Ligol." The total content of phenolic compounds varied from 1641.0 +/- 47.9 mu g/g (cv. "Ligol") to 4291.3 +/- 154.2 mu g/g (cv. "Aldas").

• 出版日期2014