Direct Electron Transfer of Glucose Oxidase and Electrocatalysis of Glucose Based on Gold Nanoparticles/Electroactivated Graphite Nanocomposite

作者：Velmurugan Murugan^*; Sakthinathan Subramanian; Chen Shen Ming; Karuppiah Chelladurai

来源：International Journal of Electrochemical Science, 2015, 10(8): 6663-6671.

• Glucose biosensor
• glucose oxidase
• gold nanoparticles
• graphite
• screen printed carbon electrode

In this study, we reported a direct electron transfer reaction of glucose oxidase (GOx) at gold nanoparticles-electroactivated graphite/screen printed carbon electrode (AuNPs-EGr/SPCE). The activated graphite was prepared through a simple electrochemical activation method in the electrolyte medium containing 0.1 M KCl. The characterization of as-prepared electrocatalyst AuNPs-EGr was studied by scanning electron microscopy and elemental analysis. The enzyme GOx was immobilized on the surface of AuNPs-EGr modified SPCE by the drop casting method. The redox behavior of GOx/AuNPs-EGr/SPCE was clearly observed at a formal potential of -0.404 V with a peak separation (Delta E-p) of 42 mV which reveals that the fast electron transfer process has been observed between GOx and AuNPs-EGr modified SPCE. The modified electrode displayed very good linear response to glucose oxidation from 50 to 1600 mu M with detection limit 2.5 mu M and the sensitivity is 255 mu AmM-1 cm(-2). The reported sensor exhibits a super selectivity and satisfactory reproducibility with good stability.

• 出版日期2015-8