Iterative Correction of Reference Nucleotides (iCORN) using second generation sequencing technology

作者：Otto Thomas D^*; Sanders Mandy; Berriman Matthew; Newbold Chris

来源：Bioinformatics, 2010, 26(14): 1704-1707.

DOI：10.1093/bioinformatics/btq269

Motivation: The accuracy of reference genomes is important for downstream analysis but a low error rate requires expensive manual interrogation of the sequence. Here, we describe a novel algorithm (Iterative Correction of Reference Nucleotides) that iteratively aligns deep coverage of short sequencing reads to correct errors in reference genome sequences and evaluate their accuracy.
Results: Using Plasmodium falciparum (81% A + T content) as an extreme example, we show that the algorithm is highly accurate and corrects over 2000 errors in the reference sequence. We give examples of its application to numerous other eukaryotic and prokaryotic genomes and suggest additional applications.

• 出版日期2010-7-15