Design of Cytocompatible Biointerfaces Based on Phospholipid Polymers to Standardize Cells and to Contribute to Cell Engineering

作者：Konno Tomohiro^*

来源：Kobunshi Ronbunshu, 2012, 69(10): 555-566.

• Biointerfaces
• Intermolecular Interaction
• Phospholipid Polymer
• Hydrogel
• Cell Engineering

Cytocompatible biointerfaces were designed to control the cell functions during cell culture period. Phospholipid polymers bearing phenylboronic acid moiety were designed to bind with cis-diol compounds such as glycoproteins for cell adhesion and poly(vinyl alcohol) (PVA) for spontaneously gelation. The phospholipid polymer surface with phenylboronic acid moieties could induce the selective adsorption of fibronectin and immunoglobulin G. The mesenchymal stem cells were adhered to the phospholipid polymer surface with a spherical shape, and the adhered cells were highly responded to differentiation reagent. The reversible polymer hydrogels were prepared by mixing with the phospholipid polymer bearing phenylboronic acid moieties and PVA. The storage modulus of the hydrogels could be controlled by the polymer ratio. The polymer hydrogel could immobilize the cells without any reduction of the bioactivity, and the cell-cycles of immobilized cells were synchronized during the preservation period in the hydrogel. We conclude that the cytocompatible biointerfaces can control standardized cells for cell engineering.

• 出版日期2012-10