Determination of alpha-chaconine and alpha-solanine in commercial potato crisps by QuEChERS extraction and UPLC-MS/MS

作者：Liu Wei; Zhang Nan; Li Bing; Fan Sai; Zhao Rong; Li Li Ping; Wu Guo Hua^*; Zhao Yao

来源：CHEMICAL PAPERS, 2014, 68(11): 1498-1504.

DOI：10.2478/s11696-014-0617-8

• steroidal glycoalkaloids
• potato crisps
• alpha-chaconine
• alpha-solanine
• QuEChERS
• UPLC-MS/MS

A simple and fast analytical method for the determination of the main steroidal glycoalkaloids, alpha-chaconine and alpha-solanine, in commercial potato crisps, based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction and ultra performance liquid chromatography coupled with an electrospray ionization triple-quadrupole tandem mass spectrometer (UPLC-MS/MS) in the multiple reaction monitoring mode was established and validated. The sample preparation procedure involves the extraction of the analytes with acidified acetonitrile and simultaneous liquid-liquid partitioning achieved by an addition of anhydrous magnesium sulfate and sodium acetate without any further clean-up steps. The limits of quantification (LOQs) for alpha-chaconine and alpha-solanine were 31 mu g kg(-1) and 16 mu g kg(-1) of fresh mass, respectively, at the signal-to-noise ratio (S/Ns) of 10. The method was applied in a survey of the content of alpha-chaconine and alpha-solanine in twenty commercial potato crisps from different brands. The results showed that all the products contained alpha-chaconine and alpha-solanine in widely varying concentrations. The amount of alpha-chaconine was higher than that of alpha-solanine in all samples.

• 出版日期2014-11
• 单位北京市预防医学研究中心