Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4

作者：Iwata Kenichi; Azlan Azlin; Yamakawa Hironori; Omori Toshio^*

来源：Journal of Bioscience and Bioengineering, 2010, 110(4): 415-418.

DOI：10.1016/j.jbiosc.2010.05.006

• Ammonia accumulation
• Lysobacter
• Nitrogen-fixing bacteria

This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30 degrees C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (similar to 1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.

• 出版日期2010-10