A potential economical substrate for large-scale production of Bacillus thuringiensis var. kurstaki for caterpillar control

作者：Paul Bishwajeet; Paul Sangeeta^*; Khan Md Aslam

来源：Biocontrol Science and Technology, 2011, 21(11): 1363-1368.

DOI：10.1080/09583157.2011.605942

• Bacillus thuringiensis var. kurstaki
• Spodoptera litura
• parthenium medium
• toxicity
• larva

Bacillus thuringiensis var. kurstaki has been widely used in caterpillar control programs. Large-scale production of this bacterium is expensive because of the high cost of the raw materials used in the medium. In this study, we attempted to develop an economical medium, based on inexpensive, locally available raw materials using a 3-L fermenter. Parthenium hysterophorus L. extract based culture medium resulted in highest toxicity (LC50 14.628 mu g mL(-1)) against 7-day-old Spodoptera litura (Fab) larvae, spore count (4.1 x 10(9) spores mL(-1)) and biomass (4.9 g L-1) within a short fermentation time of 36 h. It was 512 times cheaper than the nutrient broth (standard medium) used for B. thuringiensis production. Hence, this parthenium extract based culture medium was considered most economical with potential for the large-scale industrial production of B. thuringiensis.

• 出版日期2011