ERIODICTYOL DECREASES REACTIVE OXYGEN SPECIES PRODUCTION AND INHIBITS P47(PHOX) CLUSTERING AND TRANSLOCATION IN MONOCYTES

作者：Huang Tzou Chi; Chang Jen Yu; Liu Hung Jen; Tsai Shinn Shyong; Chang Tsung Chou; Ho Chi Tang; Chuang Kuo Pin^*

来源：Journal of Food Biochemistry, 2013, 37(4): 418-424.

DOI：10.1111/j.1745-4514.2011.00645.x

Flavonoids have been proposed to have anti-inflammatory activity. Reactive oxygen species (ROS) are important mediators in the inflammatory response. We examined the effects of luteolin, quercetin and eriodictyol on ROS production in phorbol-12-myristate-13-acetate (PMA)-stimulated U937 cells. Eriodictyol inhibited ROS production at a lower inhibition concentration 50% (IC50) then luteolin and quercetin (eriodictyol:0.822 mu M, quercetin:1.134 mu M, luteolin:1.026 mu M). Moreover, eriodictyol inhibited ROS production in a dose- and time-dependent manner. Moreover, the p47(phox) subunit of nicotinamide adenine dinucleotide phosphate oxidase regulated PMA-mediated ROS production. After eriodictyol treatment, p47(phox) clustering and translocation also decreased on stimulation of U937 cells with PMA. (phox) clustering and translocation and ROS production in monocytes - evidence that eriodictyol is an immune regulator of ROS production during inflammatory responses.

• 出版日期2013-8