An air-liquid interphase approach for modeling the early embryo-maternal contact zone

作者：Chen S; Palma Vera S E; Langhammer M; Galuska S P; Braun B C; Krause E; Lucas Hahn A; Schoen J^*

来源：Scientific Reports, 2017, 7(1): 42298.

DOI：10.1038/srep42298

We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.

• 出版日期2017-2-9