Decreased mRNA expression of CCL5 [RANTES] in Alzheimer%26apos;s disease blood samples

作者：Kester Maartje I^*; van der Flier Wiesje M; Visser Allerdien; Blankenstein Marinus A; Scheltens Philip; Oudejans Cees B

来源：Clinical Chemistry and Laboratory Medicine, 2012, 50(1): 61-65.

DOI：10.1515/CCLM.2011.731

• Alzheimer%26apos
• s disease
• blood
• inflammation
• mRNA

Background: A recent study reported that an 18-analyte multiplexed plasma panel of signaling proteins differentiated Alzheimer%26apos;s disease (AD) from controls. This study measured mRNA expression for nine of these promising bio-markers in 23 AD patients and 23 age- and sex-matched controls. %26lt;br%26gt;Methods: Total RNA was isolated from PaxGene RNA tubes. Relative mRNA expression levels of CCL5 [RANTES], CSF1, ICAM1, IGFBP6, ILIA, IL3, IL8, PDGFB and TNF were determined by Q-RT-PCR, with GAPDH as housekeeping gene. %26lt;br%26gt;Results: A panel of five markers (CCL5, CSFI, ICAM1, IL8, TNF) with detectable expression levels in all individuals differed between AD patients and controls (p interaction %26lt;0.10). Especially, the relative expression level of CCL5 was lower in AD patients than in controls (p%26lt;0.005). Across groups, levels of both CCL5 and TNF were correlated to CSF levels of tau (r =0.39, r=0.32), p tau-181 (r=0.38, r=0.33), and MMSE (r=-0.31, r=-0.33, all p%26lt;0.05). %26lt;br%26gt;Conclusions: The measured panel, and especially CCL5, could aid in the differentiation of AD from controls.

• 出版日期2012