Development of an indirect ELISA using recombinant ompH protein for serological detection of Riemerella anatipestifer infection in ducks

作者：Gao, Qun; Tang, Tian; Cheng, Anchun^*; Wang, Mingshu; Jia, Renyong; Zhu, Dekang; Chen, Shun; Liu, Mafeng; Sun, Kunfeng; Yang, Qiao; Wu, Ying; Chen, Xiaoyue; Zhang, Xin

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(2): 1330-1337.

• Riemerella anatipestifer
• omph
• elisa

Riemerella anatipestifer (RA), the causative agent of duck septicemia, results in high mortality of ducklings and causes great economic losses in duck raising industry. The outer membrane protein H (OmpH) of RA, expressed in E. coli strain BL21, was first used as coating antigen to develop an indirect ELISA assay for serological detection of RA infection. The sensitivity test indicated that the detection limit of the OmpH protein based ELISA assay (OmpH-ELISA) was twice higher than that of the OmpA protein based ELISA assay (OmpA-ELISA), and 2600 times higher than that of the conventional tube agglutination (TA) assay. In addition, the OmpH-ELISA assay displayed no cross-reaction with E. coli, S. anatum, duck hepatitis virus (DHV), avian influenza virus (AIV), Pasteurellamultocida (P. multocida) and duck plague virus (DPV) antisera, while positive with RA ATCC and RA CH-2 antisera. For detection of clinical serum samples, 115 of 156 samples were positive by OmpH-ELISA (73.7%), 105 serum samples (67.3%) were positive by OmpA-ELISA assay, only 89 serum samples (57.05%) were detected positively by TA assay. All of these features suggested that the OmpH protein is a conserved protein among different serotypes of RA, and could be used as an ELISA coating antigen for serological detection of RA infection.

• 出版日期2016
• 单位四川大学; 四川农业大学