Fast volumetric imaging of ethanol metabolism in rat liver with hyperpolarized [1-13C]pyruvate

作者：Josan Sonal^*; Spielman Daniel; Yen Yi Fen; Hurd Ralph; Pfefferbaum Adolf; Mayer Dirk

来源：NMR in Biomedicine, 2012, 25(8): 993-999.

DOI：10.1002/nbm.2762

• hyperpolarized 13C
• dynamic metabolic imaging
• fast volumetric imaging
• liver ethanol
• spiral MRSI

Rapid volumetric imaging of hyperpolarized 13C compounds allows the real-time measurement of metabolic activity and can be useful in distinguishing between normal and diseased tissues. This work extends a fast two-dimensional undersampled spiral MRSI sequence to provide volumetric coverage, acquiring a 16?x?16?x?12 matrix with a nominal isotropic resolution of 5?mm in 4.5?s. The rapid acquisition enables a high temporal resolution for dynamic imaging. This dynamic three-dimensional MRSI method was used to investigate hyperpolarized [1-13?C]pyruvate metabolism modulated by the administration of ethanol in rat liver. A significant increase in the pyruvate to lactate conversion was observed in the liver as a result of the greater availability of NADH (nicotinamide adenine dinucleotide, reduced form) from ethanol metabolism.

• 出版日期2012-8