Activation of a calcium-dependent protein kinase involved in the Azospirillum growth promotion in rice

作者：Ribaudo Claudia M^*; Cura Jose A; Cantore Maria L

来源：World Journal of Microbiology and Biotechnology, 2017, 33(2): 22.

DOI：10.1007/s11274-016-2186-1

• Azospirillum
• Rice
• CDPK
• Lateral roots growth promotion

Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2+-dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western-blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family. The fact that the amount of the protein did not change upon inoculation seems to indicate that a post-translational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation. To our knowledge, this is the first report on the possible involvement of a Ca2+-dependent protein kinase in promotion of rice plants growth by A. brasilense.

• 出版日期2017-2