Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules

作者：Minh Dao Duong Thi; Bergstrom Gunnar; Mandenius Carl Fredrik; Bergstrom Maria; Fex Tomas; Ohlson Sten^*

来源：Analytical Biochemistry, 2014, 461: 57-59.

DOI：10.1016/j.ab.2014.05.023

• Affinity determination
• Fragment-based drug discovery
• Surface plasmon resonance
• Thrombin
• Weak affinity chromatography

In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-mu M) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (K-D) was determined from retention times, and in SPR it was determined by Langmuir isotherm fitting of steady-state responses. Results indicate a strong correlation between the two methods (R-2 = 0.995, P %26lt; 0.0001).

• 出版日期2014-9-15
• 单位南阳理工学院