Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model

作者：Park Ju Ho; Choi Ji Yeon; Son Dong Ju; Park Eun Kyung; Song Min Jong; Hellstrom Mats; Hong Jin Tae^*

来源：International Journal of Molecular Sciences, 2017, 18(4): 738.

DOI：10.3390/ijms18040738

• titrated extract of Centella asiatica
• skin inflammation
• atopic dermatitis
• NF-kappa B
• cytokine
• IgE

Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica (TECA) in a phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos: HR-1 mouse. After AD induction, 100 mu L of 0.2% and 0.4% of TECA (40 mu g or 80 mu g/cm(2)) was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression byWestern blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-kappa B activity, which were determined by electromobility shift assay (EMSA). We also measured TNF-alpha, IL-1 beta, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-kappa B activity as well as the release of TNF-alpha, IL-1 beta, IL-6, and IgE. In addition, TECA (1, 2, 5 mu g/mL) potently inhibited Lipopolysaccharide (LPS) (1 mu g/mL)-induced NO production, expression of iNOS and COX-2, and NF-kappa B DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-kappa B signaling.

• 出版日期2017-4