Lectin obtained from the red seaweed Bryothamnion triquetrum: Secondary structure and anti-inflammatory activity in mice

作者：Carvalho Fontenelle Thais Pontes; Lima Glauber Cruz^*; Mesquita Jacilane Ximenes; de Souza Lopes Jose Luiz; de Brito Tarcisio Vieira; Vieira Junior Francisco das Chagas; Sales Adriano Bezerra; Aragao Karoline Saboia; Loiola Ponte Souza Marcellus Henrique; dos Reis Barbosa Andre Luiz; Ponte Freitas Ana Lucia

来源：International Journal of Biological Macromolecules, 2018, 112: 1122-1130.

DOI：10.1016/j.ijbiomac.2018.02.058

• Marine algae
• Structural conformation
• Anti-inflammatory effect

Seaweeds are sources of biomolecules with biological activities and pharmacological potential - for example, lectins, a group of proteins that can bind reversibly to carbohydrates or compounds containing them. The aim of this study was to elucidate the structural properties of a lectin extracted from the red seaweed Bryothamnion triquetrum (BtL) and to investigate its anti-inflammatory activity in mice. The lectin was purified by precipitation with ammonium sulfate and ion-exchange chromatography. Its secondary structure and tryptophan (Trp) micro environment were analyzed by circular dichroism spectroscopy and steady-state fluorescence spectroscopy, respectively. The anti-inflammatory effect was evaluated by means of paw edema induced by carrageenan or dextran, myeloperoxidase activity in paw tissue, and by measurement of leukocyte and neutrophil migration and cytokine quantification in a peritonitis model. The secondary structure of BtL is mostly composed of strands and unordered conformation, and it is quite resistant to extremes of pH and temperature, preserving the exposure of Trp residues under these conditions. In an assessment of biological activities, groups of mice were subjected to pretreatment with BtL before the inflammatory stimulus. BtL had anti-inflammatory effects in the models tested, and hence may be considered a molecule with potential to be used in the pharmaceutical industry.

• 出版日期2018-6