AEROBIC NAPHTHALENE DEGRADATION BY Ochrobactrum anthropi: KINETICS AND THERMODYNAMICS STUDY

作者：Li Bo; Wang Ban; Yang Qi^*

来源：Fresenius Environmental Bulletin, 2013, 22(4B): 1195-1202.

• Polycyclic aromatic hydrocarbons (PAHs)
• 163
• rDNA
• Biodegradation
• Monod model
• Thermodynamics

Biodegradation of naphthalene have been studied by numerous researchers. However, the kinetics and thermodynamics study of naphthalene degradation has paid less attention in current literature. An aim of our work was to provide some information about microbial degradation of naphthalene. One naphthalene degradation bacteria strain (named Na-B) was isolated from creosote-contaminated soil and used for degrade naphthalene in series of batch experiments. Based on 16S rDNA analysis, strain Na-B was closely related to Ochrobactrum anthropi. Results showed that the isolated strain were most favorable at pH 7.2, 28 degrees C. Biodegradation kinetics was described by Monod-no-growth model. The half saturation constant K(s)and the maximum specific substrate degradation rate,mu max were 9.05 mg/L and 0.020 h(-1) respectively. Furthermore, when temperature was ranged from 10 degrees C to 28 degrees C, the activation energy, activation enthalpy change and activation entropy change in biodegradation of naphthalene were 50.6 kT/mol, 48.2 kJ/mol and -172 J/ (mol.K), respectively. Thus, O. anthropi may be an alternative bacterium in degradation of naphthalene.

• 出版日期2013
• 单位中国地质大学（北京）