Hydrophobic Patch of Ubiquitin is Important for its Optimal Activation by Ubiquitin Activating Enzyme E1

作者：Singh Rajesh K; Kazansky Yaniv; Wathieu Donald; Fushman David

来源：Analytical Chemistry, 2017, 89(15): 7852-7860.

DOI：10.1021/acs.analchem.6b04194

Protein ubiquitination plays a role in essentially every process in eukaryotic cells. The attachment of ubiquitin (Ub) or Ub-like (UBL) proteins to target proteins is achieved by parallel but distinct cascades of enzymatic reactions involving three enzymes: E1, E2, and E3. The El enzyme functions at the apex of this pathway and plays a critical role in activating the C-terminus of ubiquitin or UBL, which is an essential step that triggers subsequent downstream transfer to their cognate E2s resulting in the fidelity of the Ub/UBL conjugation machinery. Despite the central role of the El enzyme in protein modification, a quantitative method to measure Ub/UBL activation by El is lacking. Here, we present a mass spectrometry-based assay to accurately measure the activation of Ub/UBL by El independent of the E2/E3 enzymes. Our method does not require radiolabeling of any components and therefore can be used in any biochemical laboratory having access to a mass spectrometer. This method allowed us to dissect the concerted process of E1-E2-catalyzed Ub conjugation in order to separately characterize the process of Ub activation and how it is affected by select mutations and other factors. We found that the hydrophobic patch of Ub is important for the optimal activation of Ub by El. We further show that the blockers of the Ub-proteasome system such as ubistatin and fullerenol inhibit Ub activation by El. Interestingly, our data indicate that the, phosphorylation of Ub at the S65 position augments its activation by the El enzyme.

• 出版日期2017-8-1