A novel on-line gold nanoparticle-catalyzed luminol chemiluminescence detector for high-performance liquid chromatography

作者：Zhang Qun Lin^*; Wu Liang; Lv Chen; Zhang Xiao Yue

来源：Journal of Chromatography A, 2012, 1242: 84-91.

DOI：10.1016/j.chroma.2012.04.036

• On-line
• Gold nanoparticle
• Chemiluminescence
• High-performance liquid chromatography
• Luminol
• Phenolic compound

A novel on-line gold nanoparticle-catalyzed luminol-H2O2 chemiluminescence (CL) detector for high-performance liquid chromatography (HPLC) was established, in which gold nanoparticles were produced by the on-line reaction of H2O2, NaHCO3-Na2CO3 (buffer solution of luminol), and HAuCl4. Eight phenolic compounds (gallic acid, protocatechuic acid, protocatechuic aldehyde, 2,5-dihydroxybenzoic acid, caffeic acid, 2,3-dihydroxybenzoic acid, (+)-catechin, and (-)-epicatechin) were chosen as the model compounds. Every separated phenolic compound in the column eluent strongly enhanced the CL signal of on-line gold nanoparticle-catalyzed luminol system. The CL and UV-visible absorption spectra and transmission electron microscopy studies were carried out, and the CL enhancement mechanism was ascribed to that the presence of phenolic compound promoted the on-line formation of 38-nm-diameter gold nanoparticles, which better catalyzed the luminol-H2O2 CL reaction. The effects of methanol and phosphoric acid in the proposed HPLC configuration were performed by two gradient elution programs, and the baseline profile revealed that on-line gold nanoparticle-catalyzed luminol-H2O2 CL detector had better compatibility than 38 nm gold colloids-luminol-H2O2 CL detector. The proposed CL detector exhibits excellent analytical performance with the low detection limit (S/N = 3) of 0.53-0.97 ng/mL (10.6-19.4 pg) phenolic compounds, and offers a new strategy for developing on-line nanoparticle-catalyzed CL detector for HPLC with sensitive analysis.

• 出版日期2012-6-15
• 单位安徽医科大学