Determination of Some Properties of Free and Immobilized Urease from Aspergillus fumigatus and Its Application in Urea Assay

作者：Tetiker Aylin Turksever^*; Ertan Figen

来源：Journal of the Chemical Society of Pakistan, 2016, 38(3): 558-564.

• Urease
• Immobilization
• Calcium alginate
• Aspergillus fumigatus
• Animal feed

Urease enzyme was extracted from Apergillus fumigatus and immobilized in calcium alginate beads. The immobilization efficiency was calculated as 82.5 %. Optimum pH and temperature for free and immobilized enzymes were found to be 7.0 and 40 degrees C, respectively. The immobilized urease had a better Km value but, catalytic efficiencies (kcat/Km) were very similar. Immobilized enzyme maintained 44% of its initial activity after 5 repeated use of enzyme. It was found that storage stability of immobilized enzyme was better than that of the free enzyme. Immobilized urease enzyme was used for the determination of urea amounts in animal feed.

• 出版日期2016-6