Alternative splicing directs two IL-20R2 isoforms and is responsible for the incomplete gene knockout via the exon I ablation

作者：Zhou, H.; Liu, X.; Yu, R.; Long, T.; Zhao, R.; Liu, H.; Xu, Y.; Liang, J. G.; Liang, P.^*

来源：Genes and Immunity, 2016, 17(4): 220-227.

DOI：10.1038/gene.2016.13

• SKIN INFLAMMATION
• RECEPTOR COMPLEX
• PSORIASIS
• IDENTIFICATION
• PATHOGENESIS
• CYTOKINES
• IMIQUIMOD
• IL-10
• MICE
• INTERLEUKIN-22

Two heterodimeric receptors consisting of interleukin (IL)-20R2 are shared by three of the IL-20 family of cytokines, IL-19, IL-20 and IL-24. Along with IL-22, these cytokines are downstream effectors of IL-23 and have been implicated in keratinocyte functions and the pathogenesis of psoriasis. Surprisingly, whereas knocking out either the IL-23 or IL-22 gene abolished imiquimod-induced psoriatic phenotypes in mice, similar attempt for IL-20R2 had little effect. Here, we report that the apparent disparity may result from a new IL-20R2 isoform encoded by an alternatively spliced transcript which survived the previous attempt for IL-20R2 gene knockout via the exon I deletion.

• 出版日期2016-6
• 单位四川大学