Anti-inflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation

作者：Boller Shirley; Soldi Cristian; Marques Maria C A; Santos Elide P; Cabrini Daniela A; Pizzolatti Moacir G; Zampronio Aleksander R; Otuki Michel F^*

来源：Journal of Ethnopharmacology, 2010, 130(2): 262-266.

DOI：10.1016/j.jep.2010.05.001

• Baccharis illinita
• Asteraceae
• Skin inflammation
• Ear oedema

Ethnopharmacologycal relevance: The tea from the leaves of Baccharis illinita DC (Asteraceae family) is commonly used by the population as anti-inflammatory (including topically), protective gastric and anti-infectious. However, no studies have been done with this species to confirm its topical anti-inflammatory action.
Aim: This study evaluated he topical effects of crude extract of leaves (CE) and its active constituents in 12-O-tetradecanoilforbol acetate (TPA)-induced ear oedema.
Methodology: CE and compounds effects were tested in commonly used models of TPA-, arachidonic acid (AA)- and capsaicin-ear oedema. Polymorphonuclear (PMN) cell migration was evaluated by mieloperoxidase and analyzed histologically.
Results: CE (0.1-1 mg/ear) caused a dose-related inhibition of TPA-induced ear oedema and PMN influx similarly to that produced by topical application of the steroidal anti-inflammatory drug dexamethasone. The active constituents of the AcOEt fraction kaurenoic acid, alpha-spinasterol, oleanolic acid and baurenol also inhibited TPA-induced ear edema. Histological analysis of the ear of CE-treated animals confirmed the reduction of edema and of PMN infiltration. Both CE and the nosteroidal anti-inflammatory drug indomethacin inhibited the AA-induced ear oedema, but did not change capsaicin-induced oedema.
Conclusion: These results indicate that the CE and the active constituents have a topical anti-inflammatory effect and the possible mechanisms for the pharmacological effects are discussed.

• 出版日期2010-7-20