Numerical impairment of nestin(+) bone marrow niches in acute GvHD after allogeneic hematopoietic stem cell transplantation for AML

作者：Medinger M^*; Krenger W; Jakab A; Halter J; Buser A; Bucher C; Passweg J; Tzankov A

来源：Bone Marrow Transplantation, 2015, 50(11): 1453-1458.

DOI：10.1038/bmt.2015.189

The nestin(+) perivascular bone marrow (BM) stem cell niche (N+ SCN) may be involved in GvHD. To investigate whether acute GvHD (aGvHD) reduces the number of N+ SCN, we examined patients with AML who had undergone allogeneic hematopoietic stem cell transplantation. In the test cohort (n = 8), the number of N+ SCN per mm2 in BM biopsies was significantly reduced in aGvHD patients at the time of aGvHD compared with patients who did not have aGvHD (1.2 +/- 0.78 versus 2.6 +/- 0.93, P = 0.04). In the validation cohort (n = 40), the number of N+ SCN was reduced (1.9 +/- 0.99 versus 2.6 +/- 0.90 N+ SCN/mm2, P = 0.05) in aGvHD patients. Receiver operating curves suggested that the cutoff score that best discriminated between patients with and without aGvHD was 2.29 N+ SCN/mm(2). Applying this cutoff score, 9/11 patients with clinically relevant aGvHD (>= grade 2) and 13/20 with any type of GvHD had decreased N+ SCN numbers compared with only 10/29 patients without clinically relevant aGvHD (P = 0.007) and 6/20 patients without any type of GvHD (P = 0.028). In patients tracked over time, N+ SCN density returned to normal after aGvHD resolved or remained stable in patients who did not have aGvHD. Our results show a decrease in the number of N+ SCN in aGvHD.

• 出版日期2015-11