A new water-soluble fluorescent Cu(II) chemosensor based on tetrapeptide histidyl-glycyl-glycyl-glycine (HGGG)

作者：Wu Shu Pao^*; Liu Shi Rong

来源：Sensors and Actuators B: Chemical , 2009, 141(1): 187-191.

DOI：10.1016/j.snb.2009.05.035

• Fluorescent chemosensor
• Cu(II) ion
• HGGG
• Fluorescein

A new water-soluble fluorescent chemosensor Fluor-HGGG was synthesized by linking a tetrapeptide histidyl-glycyl-glycyl-glycine (HGGG) with fluorescein via standard solid-phase 9-fluorenylmethoxycarbonyl (Fmoc) chemistry. Significant fluorescence quenching was observed with Fluor-HGGG in the presence of Cu(2 ). Other metal ions including Ag( ) Ca(2 ), Cd(2 ), Co(2 ), Fe(2 ), Fe(3 ), Hg(2 ), Mn(2 ), Ni(2 ) and Zn(2 ) produced only minor changes in fluorescence values for the system. The dissociation constant (K(D)) of Cu(2 ) binding in Fluor-HGGG was found to be 37 mu M. The maximum fluorescence quenching caused by Cu(2 ) binding in Fluor-HGGG was observed over the pH range 7-7.5.

• 出版日期2009-8-18