Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes

作者：Sobue Akira; Ito Norimichi; Nagai Taku; Shan Wei; Hada Kazuhiro; Nakajima Akira; Murakami Yuki; Mouri Akihiro; Yamamoto Yasuko; Nabeshima Toshitaka; Saito Kuniaki; Yamada Kiyofumi

来源：GLIA, 2018, 66(5): 1034-1052.

DOI：10.1002/glia.23299

• astrocyte
• exosome
• medial prefrontal cortex
• MHCI
• microglia

In the central nervous system, major histocompatibility complex class I (MHCI) molecules are mainly expressed in neurons, and neuronal MHCI have roles in synapse elimination and plasticity. However, the pathophysiological significance of astroglial MHCI remains unclear. We herein demonstrate that MHCI expression is up-regulated in astrocytes in the medial prefrontal cortex (mPFC) following systemic immune activation by an intraperitoneal injection of polyinosinic-polycytidylic acid (polyI:C) or hydrodynamic interferon (IFN)-gamma gene delivery in male C57/BL6J mice. In cultured astrocytes, MHCI/H-2D largely co-localized with exosomes. To investigate the role of astroglial MHCI, H-2D, or sH-2D was expressed in the mPFC of male C57/BL6J mice using an adeno-associated virus vector under the control of a glial fibrillary acidic protein promoter. The expression of astroglial MHCI in the mPFC impaired sociability and recognition memory in mice. Regarding neuropathological changes, MHCI expression in astrocytes significantly activated microglial cells, decreased parvalbumin-positive cell numbers, and reduced dendritic spine density in the mPFC. A treatment with GW4869 that impairs exosome synthesis ameliorated these behavioral and neuropathological changes. These results suggest that the overexpression of MHCI in astrocytes affects microglial proliferation as well as neuronal numbers and spine densities, thereby leading to social and cognitive deficits in mice, possibly via exosomes created by astrocytes.

• 出版日期2018-5