Arabidopsis RECEPTOR-LIKE PROTEIN30 and Receptor-Like Kinase SUPPRESSOR OF BIR1-1/EVERSHED Mediate Innate Immunity to Necrotrophic Fungi

作者：Zhang, Weiguo; Fraiture, Malou; Kolb, Dagmar; Loeffelhardt, Birgit; Desaki, Yoshitake; Boutrot, Freddy F. G.; Toer, Mahmut; Zipfel, Cyril; Gust, Andrea A.; Brunner, Frederic^*

来源：Plant Cell, 2013, 25(10): 4227-4241.

DOI：10.1105/tpc.113.117010

• PATTERN-RECOGNITION RECEPTORS
• ETHYLENE-INDUCING XYLANASE
• ELONGATION-FACTOR TU
• SCLEROTINIA-SCLEROTIORUM
• CELL-DEATH
• INTERFAMILY TRANSFER
• DISEASE RESISTANCE
• MOLECULAR-PATTERNS
• CITRX THIOREDOXIN
• DEFENSE RESPONSES

Effective plant defense strategies rely in part on the perception of non-self determinants, so-called microbe-associated molecular patterns (MAMPs), by transmembrane pattern recognition receptors leading to MAMP-triggered immunity. Plant resistance against necrotrophic pathogens with a broad host range is complex and yet not well understood. Particularly, it is unclear if resistance to necrotrophs involves pattern recognition receptors. Here, we partially purified a novel proteinaceous elicitor called SCLEROTINIA CULTURE FILTRATE ELICITOR1 (SCFE1) from the necrotrophic fungal pathogen Sclerotinia sclerotiorum that induces typical MAMP-triggered immune responses in Arabidopsis thaliana. Analysis of natural genetic variation revealed five Arabidopsis accessions (Mt-0, Lov-1, Lov-5, Br-0, and Sq-1) that are fully insensitive to the SCFE1-containing fraction. We used a forward genetics approach and mapped the locus determining SCFE1 sensitivity to RECEPTOR-LIKE PROTEIN30 (RLP30). We also show that SCFE1-triggered immune responses engage a signaling pathway dependent on the regulatory receptor-like kinases BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1 (BAK1) and SUPPRESSOR OF BIR1-1/EVERSHED (SOBIR1/EVR). Mutants of RLP30, BAK1, and SOBIR1 are more susceptible to S. sclerotiorum and the related fungus Botrytis cinerea. The presence of an elicitor in S. sclerotiorum evoking MAMP-triggered immune responses and sensed by RLP30/SOBIR1/BAK1 demonstrates the relevance of MAMP-triggered immunity in resistance to necrotrophic fungi.

• 出版日期2013-10