Amorphus orientalis sp nov., an exopolysaccharide-producing bacterium isolated from salt mine sediment

作者：Wang, Yong Xia; Liu, Ji Hui; Chen, Yi Guang; Zhang, Xiao Xia; Wang, Zhi Gang; Chen, Yun; Tian, Shi Ping; Hu, Bin; Cui, Xiao Long^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2010, 60(8): 1750-1754.

DOI：10.1099/ijs.0.015735-0

A Gram-negative, moderately halophilic, non-motile, aerobic bacterium, designated strain YIM D10(T), was isolated from a salt mine sediment sample from Yunnan, south-west China. The strain grew optimally in the presence of 3-8% NaCl and at 28 degrees C and pH 7.5. The polar lipid profile of strain YIM D10(T) comprised diphosphatidylglycerol, an unknown phospholipid and two unknown aminolipids. The major cellular fatty acids were C-18:1 omega 7c (30.5%), C-19:0 cyclo omega 8c (29.3%) and C-18:0 (13.2%). The respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content was 65.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VIM D10(T) was closely related to Amorphus coralli RS.Sph.026(T) (96.8% gene sequence similarity). Results confirmed the placement of isolate YIM D10(T) within the genus Amorphus. However, DNA DNA hybridization between strain YIM D10(T) and the type strain of the only recognized species of the genus Amorphus, A. coralli RS.Sph.026(T), was 16.7%, showing clearly that the isolate constitutes a new genospecies. Strain YIM D10(T) could be clearly differentiated from A. coralli and other phylogenetic neighbours on the basis of some phenotypic, genotypic and chemotaxonomic features. Therefore, strain YIM DIY is considered to represent a novel species of the genus Amorphus, for which the name Amorphus orientalis sp. nov. is proposed; the type strain is YIM D10(T) (=DSM 21202(T)=CCTCC AA 208035(T)).

• 出版日期2010-8
• 单位中国农业科学院农业资源与农业区划研究所; 吉首大学; 云南大学