Nephrotoxicity in Mice After Repeated Imaging Using In-111-Labeled Peptides

作者：Melis Marleen^*; Vegt Erik; Konijnenberg Mark W; de Visser Monique; Bijster Magda; Vermeij Marcel; Krenning Eric P; Boerman Otto C; de Jong Marion

来源：Journal of Nuclear Medicine, 2010, 51(6): 973-977.

DOI：10.2967/jnumed.109.074310

• nephrotoxicity
• In-111
• renal reabsorption
• radiolabeled peptide
• dosimetry

We determined the renal radiation dose of a series of In-111-labeled peptides using animal SPECT. Because the animals' health deteriorated, renal toxicity was assessed. Methods: Wild-type and megalin-deficient mice were imaged repeatedly at 3- to 6-wk intervals to quantify renal retention after injection of 40-50 MBq of In-111-diethylenetriaminepentaacetic acid-labeled peptides (octreotide, exendin, octreotate, neurotensin, and minigastrin analogs), and the absorbed kidney radiation doses were estimated. Body weight, renal function parameters, and renal histology were determined at 16-20 wk after the first scan and compared with those in naive animals. Results: Because of high renal retention, In-111-diethylenetriaminepentaacetic acid-exendin-4 scans resulted in a 70-Gy kidney radiation dose in wild-type mice. Megalin-deficient kidneys received 20-40 Gy. The other peptides resulted in much lower renal doses. Kidney function monitoring indicated renal damage in imaged animals. Conclusion: Micro-SPECT enables longitudinal studies in 1 animal. However, long-term nephrotoxic effects may be induced after high renal radiation doses, even with In-111-labeled radiotracers.

• 出版日期2010-6-1