Dopamine elevates intracellular zinc concentration in cultured rat embryonic cortical neurons through the cAMP-nitric oxide signaling cascade

作者：Hung, Hui-Hsing; Kao, Lung-Sen; Liu, Pei-Shan; Huang, Chien-Chang; Yang, De-Ming; Pan, Chien-Yuan^*

来源：Molecular and Cellular Neuroscience, 2017, 82: 35-45.

DOI：10.1016/j.mcn.2017.04.006

• Autophagy
• Dopamine
• Metallothionein
• Nitric oxide
• Protein kinase A
• Zn2+ homeostasis

Zinc ion (Zn2+), the second most abundant transition metal after iron in the body, is essential for neuronal activity and also induces toxicity if the concentration is abnormally high. Our previous results show that exposure of cultured cortical neurons to dopamine elevates intracellular Zn2+ concentrations ([Zn2+](i)) and induces autophagosome formation but the mechanism is not clear. In this study, we characterized the signaling pathway responsible for the dopamine-induced elevation of [Zn2+](i) and the effect of [Zn2+](i) in modulating the autophagy in cultured rat embryonic cortical neurons. N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), a membrane-permeable Zn2+ chelator, could rescue the cell death and suppress the autophagosome puncta number induced by dopamine. Dopamine treatment increased the lipidation level of the endogenous microtubule-associated protein 1A/1B-light chain 3 (LC3 II), an autophagosome marker. TPEN added 1 h before, but not after, dopamine treatment suppressed the dopamine-induced elevation of LC3 II level. Inhibitors of the dopamine D1-like receptor, protein kinase A (PICA), and NOS suppressed the dopamine-induced elevation of [Zn2+](i). PILE activators and NO generators directly increased [Zn2+](i) in cultured neurons. Through cell fractionation, proteins with m.w. values between 5 and 10 kD were found to release Zn2+ following NO stimulation. In addition, TPEN pretreatment and an inhibitor against PICA could suppress the LC3 II level increased by NO and dopamine, respectively. Therefore, our results demonstrate that dopamine-induced elevation of [Zn2+](i) is mediated by the D1-like receptor-PICA-NO pathway and is important in modulating the cell death and autophagy.

• 出版日期2017-7
• 单位苏州大学