A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach

作者：Lopez Girona Elena; Zhang Yu; Eduardo Iban; Hernandez Mora Jose Ramon; Alexiou Konstantinos G; Arus Pere; Jose Aranzana Maria

来源：Scientific Reports, 2017, 7(1): 6714.

DOI：10.1038/s41598-017-07022-0

<jats:title>Abstract</jats:title><jats:p>In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (<jats:italic>Ss</jats:italic>), fruits from homozygous trees (<jats:italic>SS</jats:italic>) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene <jats:italic>PRUPE.6G281100</jats:italic>, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the <jats:italic>Brassinosteroid insensitive 1-associated receptor kinase 1</jats:italic> (<jats:italic>BAK1</jats:italic>) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (<jats:italic>S</jats:italic>). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with <jats:italic>AtRLP12</jats:italic>, which may functionally complement <jats:italic>CLAVATA2</jats:italic>, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support <jats:italic>PRUPE.6G281100</jats:italic> as a candidate gene for flat shape in peach.</jats:p>

• 出版日期2017-7-27