A simple method for the simultaneous determination of mushroom toxins by liquid chromatography-time-of-flight mass spectrometry

作者：Yoshioka Naoki^*; Akamatsu Shigeki; Mitsuhashi Takao; Todo Chikage; Asano Migiwa; Ueno Yasuhiro

来源：Forensic Toxicology, 2014, 32(1): 89-96.

DOI：10.1007/s11419-013-0214-5

• Mushroom toxins
• Ibotenic acid
• Muscarine
• Amatoxins
• Phallotoxins
• LC-MS

A simple and rapid method for the determination of nine mushroom toxins, ibotenic acid, propargylglycine, choline, muscimol, muscarine, alpha-amanitin, beta-amanitin, phalloidin, and phallacidin, in mushroom samples has been developed. Mushroom toxins were extracted with 0.5 % formic acid in methanol/water, purified with Oasis HLB cartridges, and analyzed by liquid chromatography-time-of-flight mass spectrometry. The separation was performed on a pentafluorophenylpropyl column, and the mass spectrometer was operated in positive ion mode with electrospray ionization. Calibration curves were linear over the range of 0.01-1 mu g/ml (R (2) = 0.999). The detection limits for the toxins in mushroom samples were 0.0098-4.9 mu g/g, which were low enough for the investigation of food poisoning cases. The intraday and interday recoveries for blank mushroom extracts fortified with mushroom toxins were 72.5-107 % and 75.9-108 %, and relative standard deviations were %26lt; 7.9 and 8.2 %, respectively.

• 出版日期2014-1