Histopathological and Immunohistochemical Study of Air Sac Lesions Induced by Two Strains of Infectious Bronchitis Virus

作者：Bezuidenhout A; Mondal S P; Buckles E L^*

来源：Journal of Comparative Pathology, 2011, 145(4): 319-326.

DOI：10.1016/j.jcpa.2011.01.011

• air sac
• chicken
• immunohistochemistry
• infectious bronchitis virus

Infectious bronchitis virus (IBV) is a highly contagious respiratory coronavirus of domestic chickens. Although mortality is low, infection with IBV results in substantial losses for the egg and meat chicken industries. Despite the economic importance of IBV and decades of research into the pathogenesis of infection, significant gaps in our knowledge exist. The aim of this study was to compare the early progression of air sac lesions in birds receiving a vaccine strain of the virus or a more virulent field strain. The air sacs are lined by different types of epithelia and are relatively isolated from the environment, so they represent a unique tissue in which to study virus-induced lesions. Both the pathogenic and vaccine strains of the virus produced significant lesions; however, the lesions progressed more rapidly in the birds receiving the pathogenic strain. Immunohistochemistry demonstrated that in birds infected with the pathogenic strain of virus, IBV spike protein is detected first in the ciliated cells lining the air sac. These preliminary data provide important clues regarding potential mechanisms for IBV tissue tropism and spread and show that the nature of the virus isolate influences the early progression of IBV infection.

• 出版日期2011-11