A Continuous Fractionation of Ginsenosides and Polysaccharides From Panax ginseng Using Supercritical Carbon Dioxide Technology

作者：Yu I Lung; Yu Zer Ran; Koo Malcolm; Wang Be Jen^*

来源：Journal of Food Processing and Preservation, 2016, 40(4): 743-748.

DOI：10.1111/jfpp.12655

A continuous supercritical carbon dioxide (SC-CO2) fractionation was conducted to obtain ginsenosides and polysaccharides from Panax ginseng extract. P.ginseng was mixed with ethanol at a ratio of 1:4 (volume by weight) for 24h to yield ethanol extract. SC-CO2 fractionation was performed using an orthogonal design to obtain optimal fractionation parameters. The conditions were: three different temperatures (40, 50 and 60C), three different pressure levels (10, 20 and 30MPa), three flow rates of SC-CO2 (2, 4 and 6mL/min) and three flow rates of ginseng extract liquor (1, 2 and 3mL/min). The highest concentration of total ginsenosides was obtained in the fraction (F) whereas the highest concentration of total polysaccharides was retained in the residual. The highest selectivity of ginsenosides and polysaccharides were obtained at 60C and 30MPa. The greatest free radicals scavenging ability and antioxidant ability were obtained in F.
Practical ApplicationsGinsenosides and polysaccharides from Panax ginseng have been postulated to contribute a number of positive health effects. Traditional extraction techniques are associated with the disadvantages of solvent residues and its associated toxicity. In the present study, a continuous preparation method using supercritical fluid technology was described with the optimal temperature and pressure for the separation of P. ginseng ginsenosides and polysaccharide. Our findings can be served as a basis to facilitate future scale-up industrial applications.

• 出版日期2016-8