A correlation between pH and fluorescence lifetime of 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in vivo and in vitro

作者：Nakabayashi, Takakazu; Wang, Hui-Ping; Tsujimoto, Kazuo; Miyauchi, Seiji; Kamo, Naoki; Ohta, Nobuhiro^*

来源：Chemistry Letters, 2007, 36(2): 206-207.

DOI：10.1246/cl.2007.206

• INTRACELLULAR PH
• HALOBACTERIA
• MICROSCOPY

The pH dependence of the fluorescence lifetime of BCECF both in solution and in intact cells of Halobacterium salinarum has been measured with time-resolved fluorescence spectroscopy. A correlation between pH and fluorescence lifetime in vivo is different from that in vitro. The difference is ascribed to the local field produced by some protein membrane in a biological system.

• 出版日期2007-2-5
• 单位浙江大学