Acetylcholine induces mesenchymal stem cell migration via Ca2+/PKC/ERK1/2 signal pathway

作者：Tang Jun Ming^*; Yuan Jie; Li Qing; Wang Jia Ning; Kong Xia; Zheng Fei; Zhang Lei; Chen Long; Guo Lin Yun; Huang Yong Hang; Yang Jian Ye; Chen Shi You

来源：Journal of Cellular Biochemistry, 2012, 113(8): 2704-2713.

DOI：10.1002/jcb.24148

• ACETYLCHOLINE
• MUSCARINIC RECEPTORS
• MESENCHYMAL STEM CELL
• MIGRATION
• PKC
• ERK1
• 2
• CALCIUM

Acetylcholine (ACh) plays an important role in neural and non-neural function, but its role in mesenchymal stem cell (MSC) migration remains to be determined. In the present study, we have found that ACh induces MSC migration via muscarinic acetylcholine receptors (mAChRs). Among several mAChRs, MSCs express mAChR subtype 1 (m1AChR). ACh induces MSC migration via interaction with mAChR1. MEK1/2 inhibitor PD98059 blocks ERK1/2 phosphorylation while partially inhibiting the ACh-induced MSC migration. InsP3Rs inhibitor 2-APB that inhibits MAPK/ERK phosphorylation completely blocks ACh-mediated MSC migration. Interestingly, intracellular Ca2+ ATPase-specific inhibitor thapsigargin also completely blocks ACh-induced MSC migration through the depletion of intracellular Ca2+ storage. PKCa or PKC beta inhibitor or their siRNAs only partially inhibit ACh-induced MSC migration, but PKC-? siRNA completely inhibits ACh-induced MSC migration via blocking ERK1/2 phosphorylation. These results indicate that ACh induces MSC migration via Ca2+, PKC, and ERK1/2 signal pathways. J. Cell. Biochem. 113: 27042713, 2012.

• 出版日期2012-8
• 单位湖北医药学院