Comparative Study of the Immunoregulatory Capacity of In Vitro Generated Tolerogenic Dendritic Cells, Suppressor Macrophages, and Myeloid-Derived Suppressor Cells

作者：Carretero Iglesia Laura; Bouchet Delbos Laurence; Louvet Cedric; Drujont Lucile; Segovia Mercedes; Merieau Emmanuel; Chiffoleau Elise; Josien Regis; Hill Marcelo; Cuturi Maria Cristina^*; Moreau Aurelie^*

来源：Transplantation, 2016, 100(10): 2079-2089.

DOI：10.1097/TP.0000000000001315

Background Regulatory myeloid cell (RMC) therapy is a promising strategy for the treatment of immunological disorders such as autoimmune disease and allograft transplant rejection. Various RMC subsets can be derived from total bone marrow using different protocols, but their phenotypes often overlap, raising questions about whether they are truly distinct.
Methods In this study, we directly compared the phenotype and function of 3 types of RMCs, tolerogenic dendritic cells, suppressor macrophages, and myeloid-derived suppressor cells, generated in vitro from the same mouse strain in a single laboratory.
Results We show that the 3 RMC subsets tested in this study share some phenotypic markers, suppress T cell proliferation in vitro and were all able to prolong allograft survival in a model of skin transplantation. However, our results highlight distinct mechanisms of action that are specific to each cell population.
Conclusions This study shows for the first time a side-by-side comparison of 3 types of RMCs using the same phenotypic and functional assays, thus providing a robust analysis of their similarities and differences.

• 出版日期2016-10