Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection

作者：Sun, Hongxiang^*; He, Shuwang; Shi, Minghua

来源：International Immunopharmacology, 2014, 22(2): 346-355.

DOI：10.1016/j.intimp.2014.07.021

• Albizia julibrissin
• Saponin
• Adjuvant
• Active fraction
• Mechanisms
• Cytokines and chemokines

The total saponin from the stem bark of Albizia julibrissin (AJS(t)) has previously showed the adjuvant potentials in mice. In this study, AJS(t) was subjected to resin column chromatography to afford four fractions (AJS(30), AJS(50), AJS(75) and AJS(95)), and these fractions were further compared for the hemolytic activities and adjuvant potentials on the immune response to ovalbumin (OVA) and recombinant fowl pox virus vector-based avian influenza vaccine (rFPV). AJS(t), AJS(99), AJS(75) and AJS(95) showed a slight hemolytic effect. AJS(t), AJS(50) and AJS(75) significantly enhanced not only the concanavalin A (Con A)-, lipopolysaccharide (LPS)- and antigen-stimulated splenocyte proliferation, but also serum antigen-specific IgG, IgG1, IgG2a and IgG2b antibody titers in the mice immunized with OVA and rFPV. AJS(t), AJS(50) and AJS(75) also significantly promoted the NK cell activity and delayed-type hypersensitivity (DTH) in the OVA-immunized mice. Furthermore, the mechanisms of adjuvant action were explored by determining the effects of AJS(75) on cytokines and chemokines at the site of injection using antibody array. AJS75 induced or up-regulated the protein expression of 12 cytokines (IL-12p40, IL-12p40/p70, IFN-gamma, IL-13, IL-1 beta, IL-6, IL-10, TNF-alpha, sTNFR I, sTNFR III, IL-3 and IL-9) and 10 chemokines (Eotaxin, I-TAC, MIG, MIP-1 alpha, RANTES, TECK, Fracatlkine, Fast, M-CSF and GM-CSF) in the injected muscles. The results suggested that AJS(75), the most adjuvant-active fraction of AJS(t), could improve antigen-specific both cellular and humoral immune responses and simultaneously elicit a Th1/Th2 response by inducing cytokine and chemokine at the site of injection.

• 出版日期2014-10
• 单位浙江大学