摘要
The effects of chronic administration of testosterone on alpha(2)-adrenoceptor expression in male hamsters were investigated in order to explore the selectivity of testosterone regulation towards the alpha(2)-adrenoceptor subtypes. A homogeneous population of alpha(2)-adrenoceptors was identified with [H-3]RX821002 binding in adipocytes, colocytes and liver, whereas the alpha(2)-adrenoceptor sites identified in kidney and brain were heterogeneous. Competition studies with alpha(2)-adrenoceptor ligands characterized the presence of the alpha(2A)-adrenoceptor subtype in adipocytes, colocytes, kidney and brain homogenates and of the alpha(2B)-adrenoceptor subtype in kidney and liver. RNase protection assay with a selective hamster alpha(2A)-adrenoceptor cRNA probe confirmed the expression of alpha(2A)-adrenoceptor mRNA in adipocytes, colocytes, kidney and brain. Testosterone treatment did not modify the alpha(2)-adrenoceptor densities whatever the subtype, except for the adipocyte alpha(2A)-adrenoceptor, which was significantly increased. These results demonstrate that testosterone only up-regulates the adipocyte alpha(2A)-adrenoceptor.
- 出版日期1994-9-15