Association between alterations in global DNA methylation and predisposing factors in diabetes: a high pressure liquid chromatography based study

作者：Maghbooli Z; Hossein Nezhad A^*; Larijani B; Pasalar P; Keshticar A A

来源：Minerva Medica, 2015, 106(4): 221-231.

• Epigenesis, genetic
• DNA methylation
• Diabetes mellitus, type 2
• Chromatography
• high pressure liquid

Aim. The aim of this study was to investigate the relationship between inter-individual global DNA methylation and diabetes predisposing factors.
Methods. The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood leukocytes obtained from 178 type 2 diabetes patients to determine individual global DNA methylation status.
Results. There was a positive significant correlation between diabetes duration and DNA methylation levels (P=0.002) with increasing levels of DNA methylation associated with age (P=0.047). There was no significant correlation between DNA methylation levels and HbA1c (P=0.15). No significant differences were observed between patients with and without diabetes predisposing factors including: hypertension (P=0.772), dyslipidemia (P=0.617), insulin resistance (homeostatic model assessment index) (P=0.156) and obesity (P=0.609). As such, the duration of diabetes (>10 years) was the most important predictor of global DNA methylation levels in diabetic patients after adjusting for age and sex (P=0.023).
Conclusion. Our findings indicate that chronic hyperglycemic exposure plays an independent role in global DNA methylation levels in type 2 diabetes patients.

• 出版日期2015-8