Melatonin delivery by nanocapsules during in vitro bovine oocyte maturation decreased the reactive oxygen species of oocytes and embryos

作者：Remiao Mariana Harter; Lucas Caroline Gomes; Domingues William Borges; Silveira Tony; Barther Nathaniele Nebel; Komninou Eliza Rossi; Basso Andrea Cristina; Jornada Denise Soledade; Ruver Beck Ruy Carlos; Pohlmann Adriana Raffin; Varela Junior Antonio Sergio; Seixas Fabiana Kommling; Campos Vinicius Farias; Guterres Silvia Staniscuaski; Collares Tiago

来源：Reproductive Toxicology, 2016, 63: 70-81.

DOI：10.1016/j.reprotox.2016.05.016

• Antioxidant
• Bovine oocyte
• In vitro maturation
• Melatonin
• Lipid-core
• Nanocapsules
• Nanotechnology

In this work, a promising approach to increase the advantageous properties of melatonin through its encapsulation into lipid-core nanocapsules (LNC) was examined. Oocytes were treated during in vitro maturation with non-encapsulated melatonin (Mel), melatonin-loaded lipid-core nanocapsules (Mel-LNC), and unloaded LNC. Cytotoxicity, meiotic maturation rate, development to the blastocyst stage, reactive oxygen species (ROS) and glutathione levels, mean cell number and apoptotic cell/blastocyst, and mRNA quantification were evaluated. Both Mel and Mel-LNC enhanced in vitro embryo production, however, Mel-LNC proved to be more effective at decreasing ROS levels and the apoptotic cell number/blastocyst, increasing the cleavage and blastocyst rates, up-regulating the GPX1 and SOD2 genes, and down-regulating the CASP3 and BAX genes. Mel-LNC could penetrate into oocytes and remain inside the cells until they reach the blastocyst stage. In conclusion, when melatonin was encapsulated in LNC and applied during in vitro oocyte maturation, some quality aspects of the blastocysts were improved.

• 出版日期2016-8