Multi-channel cell co-culture for drug development based on glass microfluidic chip-mass spectrometry coupled platform

作者：Wu, Jing^*; Jie, Mingsha; Dong, Xueling; Qi, Hongbin; Lin, Jin-Ming^*

来源：Rapid Communications in Mass Spectrometry, 2016, 30: 80-86.

DOI：10.1002/rcm.7643

• DROPLET-BASED MICROFLUIDICS
• ELECTROSPRAY-IONIZATION
• HUMAN LIVER
• LIQUID-CHROMATOGRAPHY
• DEVICE
• METABOLISM
• ASSAY
• QUANTIFICATION
• IDENTIFICATION
• CYTOTOXICITY

RATIONALE: Cell-based drug assay plays an essential role in drug development. By coupling a microfluidic chip with mass spectrometry (MS), we developed a multifunctional platform. Cell co-culture, cell apoptosis assay, fluorescence and MS detection of intracellular drug absorption could be simultaneously conducted on this platform.
METHODS: Three micro-channels were fabricated through photolithography technology to conduct the cell co-culture. Cell apoptosis after drug treatment was assayed by fluorescent probes (Hoechst 33342). Intracellular Dox absorption was analyzed by confocal fluorescent microscopy. With a high voltage (similar to 4.5 kV) applied onto the microfluidic chip, the ionization spray was successfully generated by dropping isopropanol onto it. By coupling with a Shimadzu LCMS-2010 A mass spectrometer, intracellular CPA absorption was detected on the microfluidic chip.
RESULTS: The microfluidic chip-MS coupled platform showed high biocompatibility. Distinction of cell apoptosis between co-cultured and mono-cultured cells was detected. The results of intracellular drug absorption well explained the different cell apoptosis rate.
CONCLUSIONS: Cell-based drug assay was facilely and successfully conducted on the microfluidic chip-MS coupled platform. This technology we have devised could promote MS application in the field of drug development.

• 出版日期2016-8
• 单位中国地质大学（北京）; 清华大学